Determination of organophosphate ester flame retardants and plasticisers in fish samples by QuEChERs followed by gas chromatography-tandem mass spectrometry. Exposure and risk assessment through fish consumption

Catégorie : Impacts de la Pollution Plastique
Date :17 septembre 2020
Castro, Oscar; Pocurull, Eva; Borrull, Francesc.
Journal of Chromatography a : 1626
The presence of organophosphate esters (OPEs) in everyday commodities such as furniture, household appliances and baby toys have rendered these contaminants ubiquitous in environmental fates such as air, water, soils and biota. Their presence in food-related species suggests that an additional route of exposure to these esters for the general population is fish intake through diet. Their incipient toxicity and carcinogenetic behaviour make it essential to develop methods for determining OPEs in fish samples. In this paper we have developed a new method for determining 9 OPEs based on the QuEChERS extraction method followed by a simple clean-up using a novel device for selective lipid removal (LipiFiltr) and GC-MS/MS to extract these compounds from fish samples regardless of lipid content. QuEChERS salt packet optimisation and clean-up strategies such as liquid-liquid extraction, dispersive-solid phase extraction and LipiFiltr were tested. Our results showed that EN 15662 method salts and Lipifiltr were the best combination to produce efficient analyte apparent recovery (67-116%) and negligible matrix effects (<10%). Limits of detection ranged from 0.05 ng g(-1) (dry weight) for TiBP and TBP to 2.00 ng g(-1) (dry weight) for TCEP. Fish samples from four fish species were determined with a median concentration of Sigma OPEs 5.31 ng g(-1) on a wet weight basis, with TBP, TiBP and TCPP as the main contenders. Estimates of exposure and risk associated with consuming these compounds via dietary intake showed low levels of concern for the population of Tarragona. (c) 2020ElsevierB.V. Allrightsreserved.